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. 2019 Jul 8;6:22. doi: 10.1186/s40580-019-0192-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 7 — In vivo fluorescence imaging of FUD and 10–40 kDa PEG-FUD remaining dose after s.c. administration of a dose containing the peptide and its sulfo-Cy5 conjugate between the shoulder blades of the mouse. A blue circle drawn between the shoulder blades of each animal indicates regions of interest (ROI) used to indicate the location of the dose to quantify the total remaining dose. Same scale (8.75 × 10⁷–1.57 × 10¹⁰) is used to visualize the fluorescence intensity in all images