Table 1.
Extracted isothermal titration calorimetry (ITC) experiment binding parameters
| Interaction | [FN] μM |
[Peptide] μM |
n | Kd nM |
∆G kcal mol−1 |
∆H kcal mol−1 |
∆S cal mol−1 |
|---|---|---|---|---|---|---|---|
| FUD-Cy5:FN | 2.7 | 29.2 | 1.48 (± 0.01) | 4.7 (± 0.1) | − 11.37 (± 0.02) | − 35.8 (± 0.2) | − 82.1 (± 0.5) |
| 20 K PEG-FUD-Cy5:FN | 2.6 | 34.2 | 1.50 (± 0.01) | 13. (± 2) | − 10.8 (± 0.1) | − 35. (± 1) | − 82. (± 4) |
| FUD:FN | 2.6–2.7 | 39.2–41.4 | 1.47 (± 0.05) | 4.3 (± 0.1) | − 11.40 (± 0.02) | − 33. (± 2) | − 71. (± 6) |
| Literature [11] | [FN] μM |
[Peptide] μM |
n | Kd nM |
∆G kcal mol−1 |
∆H kcal mol−1 |
∆S cal mol−1 |
|---|---|---|---|---|---|---|---|
| FUD:FN | 1.7–1.8 | 23–29 | 1.59 (± 0.06) | 6. (± 3) | − 11.4 (± 0.3) | − 31. (± 1) | − 65. (± 3) |
| 20 K PEG-FUD:FN | 1.0–2.7 | 25–42 | 1.63 (± 0.07) | 10. (± 2) | − 10. (± 1) | − 30. (± 1) | − 66. (± 7) |