Table 1.
Sequences of primers and conditions specific for Campylobacter spp. identification in multiplex PCR
| Primer | Primer sequence (5′→3′) | Name of gene | Melting temperature (°C) | Size of amplicon (bp) |
|---|---|---|---|---|
| MD16S1 | ATCTAATGGCTTAACCATTAAC |
16SrRNA
Campylobacter jejuni + Campylobacter Coli |
51.0 | 857 |
| MD16S2l | GGACGGTAACTAGTTTAGTAT | 50.5 | ||
| MDmapA1 | CTATTTTATTTTTGAGTGCTTGTG |
mapA
C. jejuni |
50.7 | 589 |
| MDmapA2 | GCTTTATTTGCCATTTGTTTTATA | 51,2 | ||
| MDCOL3 | AATTGAAAATTGCTCCAACTATG |
ceuE
C. coli |
51.7 | 462 |
| MDCOL2 | TGATTTTATTATTTGTAGCAGCG | 51.3 |