Figure 3.

Burkholderia cenocepacia T6SS‐1 is active under standard laboratory conditions. Secretion activity of B. cenocepacia T6SS‐1 in vitro. Anti‐TssD immunoblot was performed on proteins extracted from culture supernatants (SN) and cell‐associated proteins (CA) of B. cenocepacia wild‐type (WT) strains H111, K56‐2, and Pc715j, and corresponding T6SS‐1 mutants (tssA::Tp, tssK::Tp, tssM::Tp, and/or ΔtssM) (a) and the H111 and K56‐2 WT and ΔtssM or tssM::Tp strains carrying a complementation or empty control plasmid (pBBR1‐tssM(+) and pBBR1MCS (“pBBR1”), respectively) (b). Anti‐β‐RNAP antibody was used as an indicator of bacterial cell lysis in preparations. Scales and labels as indicated. The H111 tssA::Tp mutant was included as a control