Figure 5.

The Burkholderia cenocepacia T6SS‐1 is not required for virulence toward eukaryotes. (a) Percentage survival of C. elegans following 48‐hr (white bars, left) and 72‐hr (black bars, right) infection with the indicated B. cenocepacia H111 strains at 20°C. Twenty to 40 worms were used per condition. E. coli OP50 was used as a negative control. Each point indicates mean (n = 3), and error bars indicate SD. (b) Percentage survival of wax moth larvae following 24‐hr infection with high (1 × 10⁴) (left) and low (1 × 10²) (right) doses of B. cenocepacia K56‐2 (WT) and indicated mutant strains at 37°C. Thirty larvae were infected per condition. Uninfected (UI), heat‐killed B. cenocepacia WT (HK), and mock‐infected (PBS) controls were included. Each point indicates mean % survival (n = 3), and error bars indicate SD. (c) Zebrafish embryos were microinjected with ~100 CFU of indicated B. cenocepacia K56‐2 strains and kept at 28°C in individual wells containing E3 medium. About 20 embryos were used for determination of survival percentage over time (representative experiment shown on the left), and five embryos per indicated time point were used to determine recovery of viable B. cenocepacia K56‐2 counts (n = 5 per time point per experiment, geometric mean; right‐hand graph, showing summary of two independent experiments). ns: not significant