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. 2019 Jan 9;8(7):e00774. doi: 10.1002/mbo3.774

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© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure A6 — T6SS‐1 effector loci in the B. cenocepacia J2315 genome.Schematic representation of tssI gene clusters identified in B. cenocepacia J2315 and putative T6SS‐dependent effector‐immunity pairs encoded within the clusters (tssI genes are shown in red with C‐terminal coding sequences coloured green (DUF2345 domain), brown (metalloprotease domain) or with spheres (lipase_3 domain) as observed previously (Aubert et al., 2015). The BCAL2503 tssI gene contains a deletion that results in its fusion to a divergently orientated tssF gene and is presumably non‐functional. Genes encoding the putative effectors and associated immunity‐protein are labelled and filled‐in with coloured spheres and dots, respectively. Genes encoding recognized T6SS‐adaptor proteins, Tec/Tap‐1, DUF2169‐encoding proteins and EagR, and PAAR‐containing proteins are also labelled and filled with diagonal coloured stripes and orange horizontal stripes, respectively. tssF homologues are also shown (purple). The BCAM1464‐BCAM1465 tae4‐tai4 effector‐immunity gene pair is not associated with a tssI gene cluster.