Table A1. Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Escherichia coli strains | ||
| JM83 | F− ara Δ(lac‐proAB) rpsL ϕ80dlacZΔM15 (SmR) | (Yanisch‐Perron et al., 1985) |
| SM10(λpir) | thi‐1 thr leu tonA lacY supE recA::RP4‐2‐Tc::Mu (KmR) (λpir) | (Simon et al., 1983) |
| S17‐1(λpir) | thi proA hsdR recA RP4‐2‐tet::Mu‐1 kan::Tn7 integrant (TpR, SmR) (λpir) | (Simon et al., 1983) |
| CC118(λpir) | araD139 Δ(ara‐leu)7697 ΔlacX74 galE galK phoA20 thi‐1 rpsE argE (am) recA1 λpir rpoB (RfR) | (Herrero et al., 1990) |
| OP50 | E. coli B uracil auxotroph; food source for C. elegans | (Brenner, 1974) |
| Pseudomonas putida strains | ||
| KT2440 | Spontaneous r‐ derivative of mt‐2 | (Bagdasarian et al., 1981) |
| Burkholderia cenocepacia strains | ||
| H111 | CF isolate | (Römling et al., 1994) |
| K56‐2 | CF isolate, ET12 lineage | (Mahenthiralingam et al., 2000) |
| Pc715j | CF sputum isolate, ET12 lineage | (McKevitt et al., 1989; Darling et al., 1998) |
| H111‐tssA::Tp | H111 with tssA disrupted by dfrB2 cassette (TpR) | (Dix et al., 2018) |
| H111‐tssK::Tp | H111 with tssK disrupted by dfrB2 cassette (TpR) | This study |
| H111‐tssM::Tp | H111 with tssM disrupted by dfrB2 cassette (TpR) | This study |
| H111‐ΔtssM | H111 with an in‐frame deletion of the internal XhoI fragment of tssM | (Dix et al., 2018) |
| H111‐tagY::Tp | H111 with tagY disrupted by dfrB2 cassette (TpR) | This study |
| K56‐2‐tssA::Tp | K56‐2 with tssA disrupted by dfrB2 cassette (TpR) | This study |
| K56‐2‐tssM::Tp | K56‐2 with tssM disrupted by dfrB2 cassette (TpR) | This study |
| Pc715j‐tssA::Tp | Pc715j with tssA disrupted by dfrB2 cassette (TpR) | This study |
| Pc715j‐tssK::Tp | Pc715j with tssK disrupted by dfrB2 cassette (TpR) | This study |
| Pc715j‐tssM::Tp | Pc715j with tssM disrupted by dfrB2 cassette (TpR) | This study |
| Plasmids | ||
| pBBR1MCS | Mobilizable BHR cloning vector, pBBR1‐replicon (CmR) | (Kovach et al., 1994) |
| pBluescriptII KS (+) | General cloning vector, ColE1‐derived phagemid, lacZα MCS (ApR) | (Alting‐Mees and Short, 1989) |
| p34E‐Tp | p34E containing dfrB2 gene (ApR, TpR) | (DeShazer and Woods, 1996) |
| pSHAFT2 | Suicide vector, R6K‐derived replicon, oriT+ (ApR, CmR) | (Shastri et al., 2017) |
| pBBR1‐tssK | pBBR1MCS containing tssK from B. cenocepacia H111 cloned between HindIII and BamHI (CmR) | This study |
| pBBR1‐tssM’ | pBBR1MCS‐1 containing 1.2 kbp N‐terminal fragment of tssM from B. cenocepacia H111 cloned between XbaI and XhoI (CmR) | This study |
| pBBR1‐tssM(+) | pBBR1MCS containing full‐length tssM from B. cenocepacia H111 cloned between Acc65I and XbaI (CmR) | This study |
| pBluescriptII‐tagY | pBluescriptII containing tagY from B. cenocepacia H111 cloned between BamHI and XhoI sites (ApR) | This study |
| pBBR1‐tssK::Tp | pBBR1MCS containing tssK disrupted by dfrB2 cassette at EcoRI site in same orientation as tssK (CmR, TpR) | This study |
| pBBR1‐tssM’::Tp | pBBR1MCS containing tssM’ disrupted by dfrB2 cassette at BamHI site in reverse orientation as tssM’ (CmR, TpR) | This study |
| pBluescriptII‐tagY::Tp | pBluescriptII containing tagY disrupted by the dfrB2 cassette at ZraI site in the same orientation as tagY (ApR) | This study |
| pSHAFT2‐tssA::Tp | pSHAFT2 containing tssA::Tp allele from pBBR1‐tssA::Tp cloned between XhoI and NotI (ApR, CmR, TpR) | (Dix et al., 2018) |
| pSHAFT2‐tssK::Tp | pSHAFT2 containing tssK::Tp allele from pBBR1‐tssK::Tp cloned between XhoI and NotI (ApR, CmR, TpR) | This study |
| pSHAFT2‐tssM’::Tp | pSHAFT2 containing tssM’::Tp allele from pBBR1‐tssM’::Tp cloned between XhoI and NotI (ApR, CmR, TpR) | This study |
| pSHAFT2‐tagY::Tp | pSHAFT2 containing tagY::Tp allele from pBluescriptII‐tagY::Tp cloned between SalI and XbaI (ApR, CmR, TpR) | This study |