Fig. 2.
Effect of exposure time and concentration on PM2.5-induced pulmonary fibrosis and inflammation. (A) Representative lung sections from FA, PM2.5–3L, PM2.5–6L and PM2.5–3H exposed mice were stained with hematoxylin and eosin (H&E, Scale bar = 200 μm), Masson trichrome (Scale bar = 200 μm), and antibodies specific for macrophages (galetin-3, Gal-3) and neutrophils (brown staining). Scale bar = 100 μm. The relative collagenous fiber area (B), Gal-3 (C) or neutrophil (D) positive cell number were quantified by Image J software. Data were shown as means ± SEM. N = 4, *p < 0.05; ** indicates p < 0.01; NS, not significant. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)