Figure 5.

Effect of hCitrin-mRNA Therapy on Hepatic Citrulline Levels in the CTLN2 Mouse Model following Sucrose Challenge

Mice with different genetic backgrounds were first injected (i.v.) with LNP containing either EGFP (Ctrl) or hCitrin-mRNAs (hCitrin-mRNA_CoV) (0.5 mg/kg). 24 h post-injection, the mice were challenged by oral administration of the indicated test solution: pure water (Water) or 50% sucrose solution (Sucr.) at 20 mL/kg body weight. One hour following the sucrose challenge, the mice were euthanized, and livers were collected for citrulline analysis by LC-MS. WT, wild-type mice; dKO, mice with deletion for both mitochondrial glycerol-3 phosphate dehydrogenase (mG3PD) and citrin. The data are represented as mean ± SEM (n = 3–4 per group). One-way ANOVA and Dunnett’s t test were performed to determine a statistically relevant difference between the groups. ***p < 0.001.