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. 2019 Apr 25;27(7):1252–1261. doi: 10.1016/j.ymthe.2019.04.018

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Figure.1 — Design, Construction, and Characterization of RNA Nanoparticles Carrying Anti-miR21 and the CD133 Aptamer

(A) 2D structure of 3WJ/CD133_apt/anti-miR21 (blue underline sequence is 8 nt LNA-modified anti-miR21; green sequence is 19 nt CD133 aptamer; red star is Alexa647). (B) Stepwise assembly of 3WJ/CD133_apt/anti-miR21 nanoparticles assayed by 12% TBE native PAGE. (C) Size distribution of 3WJ/CD133_apt/anti-miR21 nanoparticles. (D) Zeta-potential of 3WJ/CD133_apt/anti-miR21 nanoparticles. (E) Serum stability curve of the nanoparticles. (F) TGGE study for T_m estimation.