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. 2019 Apr 27;27(7):1286–1298. doi: 10.1016/j.ymthe.2019.04.019

Figure 5.

Figure 5

SERPINB2 Expression Was Enhanced with Aging Both In Vitro and In Vivo

(A) Schematic representation describing the functions of SERPINB2 with aging. (B) After albumin/immunoglobulin depletion, the proteins in the serum samples from young and aged mice were precipitated with 10% TCA and subjected to western blotting using an anti-SERPINB2 polyclonal antibody. (C) Stem cells from mouse adipose tissue were isolated from young (5-week-old) or aged (72-week-old) mice, and then the protein levels of SERPINB2 were evaluated using real-time PCR and western blotting, respectively. Brain (D), kidney (E), liver (F), and uterus (G) were isolated from young (5-week-old) or aged (72-week-old) mice, and then both mRNA and protein levels of SERPINB2 were evaluated using real-time PCR and western blotting, respectively. (H) The GEO database (https://www.ncbi.nlm.nih.gov/geo/) was analyzed to further verify the increased SERPINB2 expression with aging in multiple organs. β-actin was used as an internal control. The results represent the means ± SD from three independent experiments.