Figure 6.

Enhanced SERPINB2 Expression Is Associated with the Aging Process in Clinical Big Data

Clinical big data were analyzed using the Seiber dataset (GEO: GSE43996 and GSE9452) from R2: Genomics Analysis and Visualization Platform (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi/). (A) The gene datasets were filtered by the expression profiles of SERPINB2 in young patients (16) versus aged patients (17) or nonsenescent versus senescent cells. Replicative (B) and oxidative stress-mediated (C) senescence was induced by continuous subculture until passage 10 and 700 nM hydrogen peroxide (H₂O₂) exposure for 1 h, respectively. (D) Cells were stained with antibodies that were specific for SERPINB2. Multiple tissues, such as brain, kidney, liver, and uterus, from young (5-week-old) and aged (72-week-old) mice were stained with antibodies that were specific for SERPINB2. DAPI staining was used to label the nuclei within each field. The results represent the means ± SD from three independent experiments.