Table 2.
Intercorrelations between EV biomarkers and correlations with hematologic and systemic iron parameters
| Both groups combined | RLS participants | Control participants | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| nEV TfR (pg/mL) | nEV Ferroportin (pg/mL) | nEV Ferritin (ng/mL) | nEV Concentration (E10 particles/mL) | nEV TfR (pg/mL) | nEV Ferroportin (pg/mL) | nEV Ferritin (ng/mL) | nEV Concentration (E10 particles/mL) | nEV TfR (pg/mL) | nEV Ferroportin (pg/mL) | nEV Ferritin (ng/mL) | nEV Concentration (E10 particles/mL) | |||
| Spearman’s ρ | nEV TfR (pg/mL) | Correlation Coefficient | 1.000 | −.545 ** | .293 | .537 ** | 1.000 | −.535 * | .411 | .384 | 1.000 | −.624 ** | .222 | .765 ** |
| Sig. (2−tailed) | . | .000 | .074 | .001 | . | .015 | .072 | .095 | . | .006 | .376 | .000 | ||
| nEV Ferroportin (pg/mL) | Correlation Coefficient | −−.545** | 1.000 | −.247 | −.568 ** | −.535 * | 1.000 | −.215 | −.487 * | −.624 ** | 1.000 | −.300 | −.721 ** | |
| Sig. (2−tailed) | .000 | . | .135 | .000 | .015 | . | .363 | .030 | .006 | . | .226 | .001 | ||
| nEV Ferritin (ng/mL) | Correlation Coefficient | .293 | −.247 | 1.000 | .116 | .411 | −.215 | 1.000 | .071 | .222 | −.300 | 1.000 | .348 | |
| Sig. (2−tailed) | .074 | .135 | . | .488 | .072 | .363 | . | .767 | .376 | .226 | . | .157 | ||
| nEV Concentration (E10 particles/mL) | Correlation Coefficient | .537 ** | −.568 ** | .116 | 1.000 | .384 | −.487 * | .071 | 1.000 | .765 ** | −.721 ** | .348 | 1.000 | |
| Sig. (2−tailed) | .001 | .000 | .488 | . | .095 | .030 | .767 | . | .000 | .001 | .157 | . | ||
| Ferritin (mg/mL) | Correlation Coefficient | .231 | −.236 | (.607 ** ) .580 ** | .041 | .435 | −.202 | .782 ** | .051 | −.112 | −.268 | (.479 *) .298 | .129 | |
| Sig. (2−tailed) | .163 | .149 | .000 | .807 | .056 | .394 | .000 | .830 | .657 | .267 | (.011) .229 | .610 | ||
| Iron (μg/dL) | Correlation Coefficient | .129 | −.185 | .326 * | .148 | .173 | −.350 | .525 * | .314 | .056 | .215 | .172 | −.079 | |
| Sig. (2−tailed) | .442 | .259 | .046 | .376 | .466 | .130 | .018 | .177 | .826 | .378 | .496 | .756 | ||
| %Saturation | Correlation Coefficient | .189 | −.176 | .343 * | .188 | .302 | −.391 | .508 * | .365 | .034 | .230 | .224 | −.020 | |
| Sig. (2-tailed) | .255 | .284 | .035 | .259 | .196 | .088 | .022 | .113 | .893 | .343 | .372 | .938 | ||
| Hemoglobin (g/dL) | Correlation Coefficient | .111 | −.103 | .431 ** | −.018 | .224 | −.085 | .567 ** | −.083 | −.043 | .018 | .258 | .075 | |
| Sig. (2-tailed) | .508 | .532 | .007 | .914 | .342 | .723 | .009 | .729 | .864 | .943 | .301 | .766 | ||
| RDW (d/dL) | Correlation Coefficient | .006 | .162 | −.169 | −.165 | .021 | .255 | −.405 | −.057 | −.054 | .099 | .055 | −.331 | |
| Sig. (2-tailed) | .972 | .324 | .311 | .322 | .930 | .278 | .077 | .811 | .831 | .687 | .828 | .179 | ||
| MCHC (g/dL) | Correlation Coefficient | −.110 | −.171 | −.103 | .163 | −.074 | −.200 | −.062 | .162 | −.128 | −.175 | .106 | .256 | |
| Sig. (2-tailed) | .511 | .298 | .540 | .328 | .756 | .397 | .795 | .494 | .614 | .473 | .676 | .304 | ||
Spearman’s ρ and 2-tailed significance are listed for both groups combined and for separate groups. Significant correlations (p < 0.05) are in bold font; trends for significant correlations (0.1 < p < 0.05) are in bold and italic font (* depicts p < 0.05; ** depicts p < 0.01). Parentheses indicate correlations including the NIA cohort of additional Controls.