Figure 2.

Single‐cell RNA sequencing (scRNA‐seq) reveals the transcriptional heterogeneity in young and old perivascular adipose tissue‐derived stromal cells (PVASCs). (a) Experimental workflow. PVAT was dissected from aortas of young or old mice and dissociated by enzymatic digestion for scRNA‐seq. (b) t‐SNE plot of scRNA‐seq data from young or old PVASCs. (c, d) Gene ontology analysis of differentiation process. (e, f) Heat map of average expression for representative genes indicative of adipogenic lineage and endothelial lineage and cell‐type classification of the main clusters from young (e) and old (f) data set. (g‐l) The adipogenic and endothelial differentiation were determined by specific staining in the young and old PVASCs. Scale bar 50 μm. n = 5 independent experiments