TABLE 1. Percent infection of bodies and heads of two strains of Aedes mosquito (based on cytopathic effect (CPE) analysis) and virus quantitation in saliva after exposure to CHIKV strain BVI 99660,a,b Fort Collins, Colorado, United States, 2016.
Mosquito strainc |
Number tested |
Infecting dose (log10 pfud/mL) |
CPE analysis |
Virus quantitation in saliva |
||
|---|---|---|---|---|---|---|
Body (%) |
Head (%) |
Pose (%) |
Titer (pfu) |
|||
Ae. aegypti (PR) |
36 |
4.6 |
13.9 |
80.0 |
0 |
0 |
|
28 |
4.9 |
14.3 |
75.0 |
0 |
0 |
|
10 |
5.3 |
60.0 |
100.0 |
33.3 |
505 |
|
37 |
5.6 |
73.3 |
100.0 |
53.3 |
935 |
|
36 |
6.1 |
91.7 |
100.0 |
75.0 |
5 000 |
|
24 |
6.3 |
95.8 |
100.0 |
36.8 |
3 545 |
Ae. albopictus (LC) |
30 |
2.9 |
0 |
0 |
0 |
0 |
|
40 |
3.9 |
35.0 |
92.9 |
66.7 |
1 921 |
|
32 |
4.6 |
71.9 |
95.7 |
66.7 |
2 334 |
|
39 |
5.7 |
84.6 |
97.0 |
94.4 |
39 100 |
Chikungunya virus (human serum sample collected in the British Virgin Islands in 2014).
All results are for Day 8 post-exposure except the 6.3 log10 dose, which was for Day 12 post-exposure.
Ae. aegypti collected in Puerto Rico in 2015 (“PR”) and Ae. albopictus collected in Lake Charles, Louisiana, United States, in 1990 (“LC”) (3- to 4-day-old adults).
Plaque-forming units.
“Positive” (proportion of CPE-positive heads with saliva positive for the virus based on quantitative reverse transcription polymerase chain reaction (qRT-PCR)).