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. 2019 Mar 12;123(7):1191–1203. doi: 10.1093/aob/mcz025

Fig. 5.

Fig. 5.

De novo shoot and leaf organogenesis in P. edulis displays visible juvenile-to-adult leaf transition under glucose treatment. (A) Leaves showing adult traits of serration of the margin and lobe formation (arrowheads) when cotyledons were cultured on glucose-supplemented medium. (B) Young leaf with monolobed blade. (C, D) Leaves in intermediate stage of development, in which both types of juvenile–adult markers are present in the same leaf. (E) Adult leaf (trilobed). Scale bar = 3 mm. (F, G) Expression patterns of (F) miR156 and (G) miR156-targeted SPL9 (PeSPL9) during de novo shoot and leaf organogenesis. Explants were cultivated in induction medium (IM) with or without 44 or 88 mm glucose for 3 or 12 d. Values are mean ± s.e. of at least two independent biological replicates. Asterisks indicate significant differences among treatments (P < 0.05).