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. 2019 Mar 12;123(7):1191–1203. doi: 10.1093/aob/mcz025

Fig. 6.

Fig. 6.

In situ hybridization reveals that SPL9 is expressed early during de novo shoot organogenesis in P. edulis. (A, B) Morphological characterization of the initiation of meristemoid formation followed by stem bud development. Stereomicroscope images. (C–F) Light microscope images. (C) Histological sections of the initiation of meristemoid formation highlighting SPL9 gene expression in epidermal and parenchyma cells during the dedifferentiation process. (D, E) Detection of PeSPL9 transcripts in the shoot apical meristem region and at leaf primordia initiation. (F) PeSPL9 transcripts detected in epidermal cells of the abaxial surface of the leaf blade (arrowhead). Abbreviations: me, meristemoid; sb, stem bud; SAM, shoot apical meristem; lp, leaf primordium. Scale bars: (A, B) = 200 μm; (C, D, F) = 150 μm; (E) = 400 μm.