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Characteristics of isolated Topaz-positive cells. (a) Sorting of Topaz-positive cells with the FACSAria II Cell Sorter. (b) Morphology of Topaz-positive cells. (c) Expression levels of DMP1 and SOST mRNA in the osteoblast-rich fraction (fraction 2) and in all Topaz-positive cells (n = 4; ∗ P < 0.05 and ∗∗ P < 0.01).
