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. 2019 Jul 1;11:5983–6001. doi: 10.2147/CMAR.S207084

Figure 2.

Figure 2

Forced expression of Linc00460 promotes proliferation and metastasis of breast cancer cells both in vitro and in vivo. (A) Expression levels of Linc00460 were determined by qRT-PCR in MCF-7 and MDA-MB-231 cells stably transfected with Linc00460 shRNAs (460-sh1 and 460-sh2) or empty vector (Ctr). (B) Expression levels of Linc00460 were determined by qRT-PCR in MCF-7 and MDA-MB-231 cells stably transfected with pSin plasmid containing Linc00460 (pSin-460oe) or the empty vector (pSin-vec). (C and E) Cell viabilities of the respective stably transfected MCF-7 and MDA-MB-231 cells were determined by MTT assay over a period of 5 days. (D and F) Colony formation of the stably transfected MCF-7 and MDA-MB-231 cells was determined over a period of 10 days. (G–J) Transwell assays of the stably transfected MCF-7 and MDA-MB-231 cells. Representative pictures were taken at 100× magnification, and the total number of cells on the bottom surface of transwell were counted and presented as the relative fold changes. In all panels of this figure, the statistical differences were all analyzed using independent samples t-test. (K-L) Growth of MCF-7-vec or MCF-7-Linc00460 cells-derived tumors in the orthotopic xenograft models (N=6). Data are presented as mean ± SEM and analyzed using two-way ANOVA. *P<0.05. (M) H&E staining of respective MCF-7-vec or MCF-7-Linc00460 cells-derived tumors. Arrow indicates fat pad invasion of MCF-7-Linc00460 cells-derived tumors. Images were taken at 100× magnification. (N) H&E staining of lung sections of mice with tail vein injections of MDA-MB-231 cells stably transfected with either Linc00460 shRNAs (460-sh1 and 460-sh2) or empty vector (Ctr). The metastatic nodules were indicated by white arrow. Images were taken at 200× magnification. (O) Incidence of lung metastasis in mice of tail vein injection model. χ2 test, **P<0.01.