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. 2019 Jun 9;18(3):247–255. doi: 10.1002/rmb2.12283

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© 2019 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Undifferentiated spermatogonium on the basement membrane in adult mouse testis. A confocal image of GFRα1+ spermatogonium (green) and nuclear (blue) in C57BL6 adult (3‐mo‐old) mouse testis. GFRα1+ cell was visualized by immunohistochemistry by using anti‐GFRα1 antibody and Alexa488‐conjugated anti‐goat IgG antibody with a nuclear staining by Hoechst 33342, following the procedures described in a previous papers.23, 32 White dotted line indicates a periphery of the seminiferous tubule. Bar indicates 10 μm