Table 2.
Comparison between controls and psoriatic skin (lesional and perilesional) regarding JAK1 and STAT3 tissue immunoreactivity
| Immunoreactivity parameters | Controls (total 26), n (%) |
Perilesional (total 26), n (%) |
Lesional (total 26), n (%) |
Test of significance |
P-value & post hoc test |
|---|---|---|---|---|---|
| Epidermal JAK1 | |||||
| Expression: | |||||
| Positive | 11 (42.3) | 21 (80.0) | 23 (88.5) | χ2=15.2 | P≤0.001 |
| Negative | 15 (57.7) | 5 (19.2) | 3 (11.5) | ||
| Localization: | |||||
| Nuclear | 0 | 0 | 2 (8.7) | Fisher’s exact = 4.6 | P=0.28 |
| Cytoplasmic | 8 (72.7) | 19 (90.5) | 16 (69.6) | ||
| Nucleocytoplasmic | 3 (27.3) | 2 (9.5) | 5 (21.7) | ||
| H score: | P1≤0.001* | ||||
| Mean ± SD | 90.8±32.3 | 130.9±3.9 | 164.7±20.4 | P2≤0.001* | |
| Median | 130 | 130 | 170 | KW=20.1 | P3≤0.001* |
| Range | 30–130 | 80–180 | 120–200 | P≤0.001* | |
| Dermal JAK1 | |||||
| Expression | |||||
| Positive | 3 (11.5) | 8 (30.8) | 19 (73.1) | χ2=21.7 | P≤0.001* |
| Negative | 23 (88.5) | 18 (69.2) | 7 (26.9) | ||
| Localization | |||||
| Nuclear | 0 | 3 (37.5) | 0 | Fisher’s exact =10.4 | P=0.03* |
| Cytoplasmic | 2 (66.7) | 5 (62.5) | 14 (73.7) | ||
| Nucleocytoplasmic | 1 (33.3) | 0 | 5 (26.3) | ||
| H score | P1=0.02* | ||||
| Mean ± SD | 50±17.32 | 86.2±32.9 | 160±18.8 | KW=32.7 | P2≤0.001* |
| Median | 40 | 85 | 160 | P3≤0.001* | |
| Range | 40–70 | 30–120 | 120–190 | P≤0.001* | |
| Epidermal STAT3 | |||||
| Expression: | |||||
| Positive | 26 (100) | 26 (100) | 26 (100) | NA | — |
| Negative | 0 | 0 | 0 | ||
| Localization: | |||||
| Nuclear | 2 (7.7) | 0 | 0 | ||
| Cytoplasmic | 0 | 0 | 0 | ||
| Nucleocytoplasmic | 24 (92.3) | 26 (100) | 26 (100) | ||
| H score | P1<0.001* | ||||
| Mean ± SD | 180.3±34.2 | 110.0±41.1 | 225.0±43.1 | P2<0.001* | |
| Median | 170 | 115 | 230 | P3<0.001* | |
| Range | 110–250 | 50–180 | 170–300 | P<0.001* | |
| Expression: | |||||
| Positive | 26 (100) | 22 (84.6) | 19 (73.1) | Fisher’s exact=7.8 | P=0.01* |
| Negative | 0 | 4 (15.4) | 7 (26.9) | ||
| Localization: | |||||
| Nuclear | 2 (7.7) | 0 | 0 | ||
| Cytoplasmic | 0 | 0 | 0 | NA | — |
| Nucleocytoplasmic | 24 (92.3) | 22 (100) | 19 (100) | ||
| H score | P1=0.43 | ||||
| Mean ± SD | 185.3±30.6 | 129.1±35.1 | 193.6±40.9 | KW=21.5 | P2<0.001* |
| Median | 170 | 120 | 280 | P3<0.001* | |
| Range | 160–250 | 80–180 | 120–280 | P≤0.001* | |
Notes: *Significant; P1, control vs lesional; P2, control vsperilesional; P3, lesional vs perilesional.
Abbreviations: NA, not applicable; KW, Kruskal–Wallis; JAK1, janus kinase 1; STAT3, signal transducer and activator of transcription.