Fig. 2.

Phenotypic and genotypic comparison of G2 offspring. (Left) WT and MLPH^+/− quail with dark brown plumage and MLPH^−/− quail with gray plumage without any change in plumage pattern. (Right) DNA sequencing chromatograms of MLPH^+/− and MLPH^−/− quail, which indicate there is a one base pair deletion of one allele and both alleles, respectively. Dashed line indicates the point where one base pair deletion occurred. The PAM sequences are highlighted in gray. Nucleotide sequences are presented in a negative direction.