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Comparison of dose-dependent changes in the total integrated intensity (I) with protein at 1 mg ml−1 irradiated in solution with different radical scavengers shows that ROS is the predominant cause of aggregation. (a) Protein irradiated in PBS alone, (b) in PBS and 10 mM ascorbic acid, (c) in PBS and 2% glycerol and (d) in PBS with 10 mM sodium nitrate.
