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. 2019 Jun 13;116(27):13508–13516. doi: 10.1073/pnas.1903165116

Fig. 4.

Fig. 4.

Challenge with a second donor-type graft breaks tolerance. Groups of mice received BALB/c skin grafts under tolerogeneic IL-2 cplx protocol. Operationally tolerant mice (graft macroscopically intact) were grafted with a second graft of BALB/c strain (A and B) at day 25 (d25) (PBS control n = 3, cplx protocol n = 5, cplx protocol + graft d25 n = 5; P = 0.0018 ± second graft) or (C and D) at d60 (PBS control n = 3, cplx protocol n = 4, second graft: cplx protocol + graft d60 n = 4; P = 0.0091 ± second graft) after primary skin grafting. Overall survival of the primary grafts with or without additional antigen challenge on (A) d25 or (C) d60 and survival of the grafts after placing a second donor-type graft transplanted on (B) d25 and (D) d60 (second graft d25 MST = 10 d; second graft d60 MST = 10.5 d; P = n.s. vs. PBS control) are shown. Log-rank test was used for comparison of survival curves.