Fig. 1.
Mtb strains encoding point mutants of CarD show genome-wide differences in gene expression. (A) Diagram showing the protein domain structure of CarD, which consists of a N-terminal RID (amino acids 1–66) and a C-terminal DBD (amino acids 67–162). The table lists the 4 CarD point mutations that were used in this study and their effects on CarD function. (B) Pie charts display the proportion of 4,016 Mtb protein encoding genes with nonzero reads that were significantly (Padj < 0.05) up-regulated (fold-change > 0), down-regulated (fold-change < 0), or not significantly differentially expressed (N.S., Padj ≥ 0.05) in each CarD mutant relative to their expression in CarDWT. The bars under FC > 2 show the distribution of up- and down-regulated genes for the subset of Mtb genes that were significantly differentially expressed greater than 2-fold in each mutant genotype. These data are enumerated in the table below. Differential expression testing was performed using RNA-seq data from 3 replicates of each genotype.