Figure 1.

Kisspeptin receptor (kisspeptin R) protein expression in human decidual stromal cells. Results show cells from three different women (EM1, 2, and 3). (A) Immunoblots, Ishikawa endometrial cancer cells served as a positive control. In the final two columns, human decidual stromal cells were transfected with scrambled siRNA (si-Ctrl) or siRNA targeting human kisspeptin R. (B) Immunohistochemistry of decidual endometrium sections; column 1, hematoxylin and eosin staining; column 2, kisspeptin (brown staining) endothelial cells (arrow) and stromal cells (arrowhead) are indicated in row 2; column 3, kisspeptin receptor (brown staining), endothelial cells (arrow), and stromal cells (arrowhead) are indicated in row 2; column 4, second antibody only control. Micrographs were taken with the 400× objective lens. Scale bars represent 20 μm.