Figure 2.

Kisspeptin agonist (K10) inhibits invasion and migration of decidual stromal cells. (A) Invasion, decidual stromal cells were seeded onto a Matrigel-precoated filter in Transwell chambers containing 0-, 100-, 300-, or 500-nM KP10. (B) Migration, decidual stromal cells were seeded on an uncoated porous filter in a transwell chamber containing 0-, 100-, 300-, or 500-nM KP10. KP10 inhibited the invasion and migration of decidual stromal cells in a dose-dependent manner with a maximal effect at 500 nM. After 48 (invasion, A) and 24 (migration, B) hours of incubation, cells on the upper side of the filter were removed and the invaded or migrated cells on the lower surface were fixed, stained with crystal violet, and counted. The photomicrographs show representative results. Bar charts show the mean number of invaded or migrated cells of four fields of triplicate wells from three independent experiments + SEM; *P < 0.05, versus control.