Table 1.
The primer sequences for RT-qPCR
| Gene | Forward primer (5ʹ–3ʹ) | Reverse primer (5ʹ–3ʹ) |
|---|---|---|
| miR-136 | GGGACUCCAUUUGUUUUGAU | CAGTGCGTGTCGTGGAGT |
| LINC01116 | TTCAAGTGCGTCCGGGTTT | CCTGCCGATTCCCCATTCC |
| U6 | CCAGCCCATGATGGTTCTGAT | GGCTGGTAAGGATGAAGG |
| FN1 | CGGTGGCTGTCAGTCAAAG | AAACCTCGGCTTCCTCCATAA |
| Vimentin | CGCCAGATGGGTGAAATGG | ACCAGAGGGAGTGAATCCACA |
| N-cadherin | ACAGTGGCCACCTACAAAGG | CCGAGATGGGGTTGATAATG |
| E-cadherin | CCCACCACGTACAAGGGTC | CTGGGGTATTGGGGGCATC |
| MMP-9 | TGTACCGCTATGGTTACACTCG | GGCAGGGACAGTTGCTTCT |
| GAPDH | ACAGTCCATGCCATCACTG | AGTAGAGGCAGGGATGATG |
Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-136, microRNA-136; FN1, fibronectin1; E-cadherin, epithelial cadherin; N-cadherin, neural cadherin; MMP-9, matrix metalloprotein 9; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.