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. Author manuscript; available in PMC: 2020 Mar 12.
Published in final edited form as: Biochemistry. 2019 Feb 20;58(10):1388–1399. doi: 10.1021/acs.biochem.8b01278

Figure 2.

Figure 2.

13C-NMR spectrum for the reaction catalyzed by 5.0 µM YcjS with 1.0 mM [UL-13C6]-d-glucose and 2.0 mM NAD+. (a) [UL-13C6]-d-glucose and NAD+ before the addition of YcjS; (b) after incubation with the enzyme. The resonance for formate is observed at 171.03 ppm.