Table 2.

: Equilibrium constants for the conversion of 9 to 3^a

Reaction	Enzyme	Keq
9 to 11	YcjQ	3.6 ± 0.2 × 10−4
11 to 7b	YcjR	3.9 ± 0.2
7 to 3c	YcjS	6.4 ± 0.3 × 103
9 to 3	YcjQ, YcjR, YcjS	9.0 ± 0.9d

^aReactions were carried out at 30 °C in 50 mM CHES/K⁺ buffer, pH 9.0 with 2.0 mM NAD⁺.

^bReaction was followed by monitoring the oxidation 9 to 11 and conversion of 11 to 7 with excess YcjR present. The equilibrium constant of 11 to 7 was calculated as described in the text.

^cReaction was followed by monitoring the oxidation of 3 to 7.

^dCalculated based on the individual equilibrium constants measured for each step of the conversion of 9 to 3.