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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 May 16;39(7):1419–1431. doi: 10.1161/ATVBAHA.118.312346

Figure 1. Nicotine induces arterial stiffness and remodeling of extracellular matrix in mice.

Figure 1.

A. Representative images of motion (M)-mode for carotid artery monitored by ultrasound from vehicle- or nicotine-treated mice. B. Representative images of M-mode for abdominal aorta monitored by ultrasound from vehicle- or nicotine-treated mice. C. Circumferential cyclic strain (upper panel) and analysis of segmental stiffness (lower panel) of carotid artery and abdominal aorta in vehicle- or nicotine-treated male mice (n=5; * P < 0.05 vs. Veh). D. Circumferential cyclic strain (upper panel) and analysis of segmental stiffness (lower panel) of carotid artery and abdominal aorta in vehicle- or nicotine-treated female mice (n=5; * P < 0.05 vs. Veh). E. Representative images and quantification of collagen content in aorta based on Masson trichrome staining (Blue color) of vehicle- or nicotine-treated mice (n=5; * P < 0.05 vs. Veh). F. Representative images and quantification of collagen I in aorta based on IHC staining of vehicle- or nicotine-treated mice (n=5; * P < 0.05 vs. Veh). G. Representative images and quantification of fibronectin in aorta based on IHC staining of vehicle- or nicotine-treated mice (n=5; * P < 0.05 vs. Veh). H. Representative images and quantification of elastin fragmentation in aorta based on Van Gieson’s staining of vehicle- or nicotine-treated mice (n=5; * P < 0.05 vs. Veh). Veh, vehicle; Nic, nicotine. Negative controls for anti-Collagen I staining and anti-Fibronectin staining were presented in Online Figure I.