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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 May 16;39(7):1419–1431. doi: 10.1161/ATVBAHA.118.312346

Figure 3. Sirt1 overexpression eliminates nicotine-induced arterial stiffness.

Figure 3.

A. Western blot analysis of SIRT1 protein expression in aortas from WT and Sirt1-overexpressing (Sirt1Super) mice treated with vehicle or nicotine for 4 weeks (n=5; * P < 0.05 vs. WT/Veh; # P < 0.05 vs. WT/Nic). B. SIRT1 activity was measured from aorta nuclear extracts. Data were normalized to the activity measured in vehicle-treated WT mice (n=5; * P < 0.05 vs. WT/Veh; # P < 0.05 vs. WT/Nic). C. Representative images of motion (M)-mode for carotid artery monitored by ultrasound from WT and Sirt1Super mice treated with/without nicotine. D. Circumferential cyclic strain of carotid artery in WT and Sirt1Super mice with/without nicotine treatment (n=10; * P < 0.05 vs. WT/Veh; # P < 0.05 vs. WT/Nic mice). E. Analysis of segmental stiffness of carotid artery (n=10; * P < 0.05 vs. WT/Veh; # P < 0.05 vs. WT/Nic mice). F. Representative images of M-mode for abdominal aorta monitored by ultrasound from WT and Sirt1Super mice treated with/without nicotine. G. Circumferential cyclic strain of abdominal aorta in WT and Sirt1Super mice with/without nicotine treatment (n=10; * P < 0.05 vs. WT/Veh; # P < 0.05 vs. WT/Nic mice). H. Analysis of segmental stiffness of abdominal aorta (n=10; * P < 0.05 vs. WT/Veh; # P < 0.05 vs. WT/Nic mice). Veh, vehicle; Nic, nicotine.