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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 May 16;39(7):1419–1431. doi: 10.1161/ATVBAHA.118.312346

Figure 6. Nicotine upregulates peroxynitrite formation in mouse aorta in vivo.

Figure 6.

A. Representative images of IHC staining and quantification of iNOS in aortas from mice with or without nicotine treatment (n=5; * P < 0.01 vs. Veh). B. Representative images of IHC staining and quantification of 3-nitrotyrosine (3-NT) in aortas from mice with or without nicotine treatment (n=10; * P < 0.01 vs. Veh). C. Representative images of immunofluoresence co-staining and quantification of 3-NT and SIRT1 in aorta from mice with or without nicotine treatment (n=10; * P < 0.05 vs. Veh). D. Representative images of IHC staining and quantification of 3-NT in aortas from mice with or without nicotine treatment along with control or Tempol treatment (n=5; * P < 0.01 vs. Veh; # P < 0.01 vs. Nic alone). E. Tempol reversed nicotine-induced SIRT1 and pYAP reduction in aortas (n=5; * P < 0.01 vs. Veh; # P < 0.05 vs. Nic alone). F. Tempol attenuated SIRT1 activity inhibition by nicotine (n=5; * P < 0.01 vs. Veh; # P < 0.05 vs. Nic alone). G. Representative images and quantification of collagen content in aortas based on Masson trichrome staining (Blue color) of mice with/without nicotine treatment along with control or Tempol treatment (n=5; * P < 0.01 vs. Veh; # P < 0.01 vs. Nic alone). Veh, vehicle; Nic, nicotine. Negative controls for staining of anti-iNOS and anti-3-NT were presented in Online Figure V.