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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Ticks Tick Borne Dis. 2019 Apr 6;10(4):838–847. doi: 10.1016/j.ttbdis.2019.04.002

Figure 3. Src tyrosine kinase levels in unfed I. scapularis nymphal ticks are reduced upon A. phagocytophilum infection.

Figure 3.

A) QRT-PCR analysis showing expression of I. scapularis Src kinase at different tick developmental stages in uninfected unfed-larvae, nymphs, adult female and adult male ticks. Each open square, open circle, and open inverted triangle or triangle represents one individual tick. B) Src kinase expression between unfed uninfected nymphs and unfed A. phagocytophilum-infected nymphs is shown. C) Immunoblot showing reduced potential Src kinase expression at the protein level in unfed uninfected ticks and, upon A. phagocytophilum-infection. The arrow denotes a prominent intensity band between 50–60 kDa (potential phosphorylated Src protein band). The Ponceau S stained image serve as control. M indicates marker, and the marker sizes are shown in kDa on the left. D) Src kinase expression between uninfected nymphs and A. phagocytophilum-infected nymphs collected at 48 h during feeding is shown. Open circles represents uninfected (UI) and closed circles represent infected (I) ticks in both (B) and (D). Each circle represents one tick. The mRNA levels of src are normalized to tick beta-actin mRNA levels. P value from non-paired Student’s t-test is shown.