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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Ticks Tick Borne Dis. 2019 Apr 6;10(4):838–847. doi: 10.1016/j.ttbdis.2019.04.002

Figure 5. A. phagocytophilum upregulates Src kinase levels in tick cells.

Figure 5.

A) QRT-PCR analysis showing A. phagocytophilum-GFP strain burden upon infection of tick cells with different bacterial doses (100, 200 and 400 μl of suspension contaning A. phagocytophilum-GFP strain prepared from infected HL-60 cells) at 24 h post infection. The uninfected (UI) cells serve as control. B) The upregulated src transcript levels are shown in the same samples treated with different doses of A. phagocytophilum-GFP strain in tick cells at 24 p.i.. Each circle represents data from one independent well of the culture plate. The mRNA levels of src and P44 (A. phagocytophilum gene) are normalized to tick beta-actin levels. P value from non-paired Student’s t-test is shown. C) Immunoblotting analysis with anti-Src antibody showing levels of Src kinase protein at approximately 49–50 kDa position in tick cells infected with A. phagocytophilum at different doses at 24 p.i. Total profile of proteins observed on Coomassie stained gel serves as a loading control.