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. 2019 Jul 2;12:1877–1887. doi: 10.2147/IDR.S210226

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© 2019 Zhong et al.

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Sensitivity of the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. (A–E) Visual detection of the LAMP amplification products of mcr-1 to mcr-5 genes with SYBR Green I. (F–J) Agarose gel electrophoresis was conducted for the LAMP products of mcr-1 to mcr-5 genes. (K–O) Comparative agarose gel electrophoresis analysis of products of the PCR assay and the corresponding LAMP assay. Lane M, Trans 2K plus II DNA marker; Lanes 1–7, serial 10-fold dilutions of templates from 10⁸ copies/µL to 10² copies/µL; Lane 8, negative (water).