Table S1.
The primer sequences of LAMP for mcr-1 to mcr-5 genes
| Target gene | Primers | Sequences (5ʹ-3ʹ) | Reference |
|---|---|---|---|
| mcr-1 (1) | F3 | TGATGCAGCATACTTCTGTG | This study |
| B3 | GACCGTGCCATAAGTGTC | ||
| FIP | GCGATGGGATAGGTTTGGCTGTGTTGCCGTTTTCTTGAC | ||
| BIP | TGCTGACGATCGCTGTCGGCACATAGCGATACGATGAT | ||
| LF | AAGGTAAGATTGGCGGTCG | ||
| LB | CTACTGATCACCACGCTGTT | ||
| mcr-1 (2) | F3 | TGCTACCAAGTTTGCTTGT | This study |
| B3 | CGTCTTTGGCGTGATAAATG | ||
| FIP | AGACTTGCCACGATCAAGCCTTAAGGTGGATTATCCGACTTG | ||
| BIP | GCGTTCAGCAGTCATTATGCCGTAGATTGGCATGATCGGAT | ||
| LF | CAATCGGCGCATCAAACC | ||
| LB | CGCTGCGTAGCTATGTCA | ||
| mcr-1 (3) | F3 | ATACCGCCAAATACCAAGAAA | This study |
| B3 | CATCTAAGCCAACGAGCAT | ||
| FIP | GCAGCTTATCCATCACGCCTTGATACGCTGGATCGCTTG | ||
| BIP | GCCGATTATAAATCCGCGACCAGCGGCATTCGTTATAAGGA | ||
| LF | GTCCGAATTATTATCACGCCAC | ||
| LB | CAACGCCATCTGCAACAC | ||
| mcr-2 (1) | F3 | ACGCCTAGTGGTGTTCGT | This study |
| B3 | GCGGTCAAGCGTATCTAGC | ||
| FIP | AACTTTGGCAAGCTGCGGGAATGCTGACCATGTGCAGTTC | ||
| BIP | TCGACGGCGTATTCTGTGCCGGTATTTGGCGGTATCGACA | ||
| LF | AGTCTCACGGCCATAGCC | ||
| LB | GTGTATGTTCAGCTATTTGGGTCA | ||
| mcr-2 (2) | F3 | GGGTAAGCTTGCCAGTATCG | This study |
| B3 | AGCTGCGGGAAAGTCTCA | ||
| FIP | CTCGGCTTGGTGGTCTGCACCACTGCGCCAACAGACAC | ||
| BIP | GAGCGTAAGCCACGCCTAGTGCGGCCATAGCCATTGAACTG | ||
| LF | GGCGTCTTTGGCATGATAGATG | ||
| LB | GTGTTCGTCGTCGGTGAGA | ||
| mcr-2 (3) | F3 | CAAAGACGCCGTGCAGAC | This study |
| B3 | CAGAATACGCCGTCGATGT | ||
| FIP | ACTGCACATGGTCAGCACGCGAGCGTAAGCCACGCCTA | ||
| BIP | GGCTATGGCCGTGAGACTTTCCGCCACACGATGTCACTTGG | ||
| LF | ACCGACGACGAACACCAC | ||
| LB | CTTGCCAAAGTTGATGGCTTG | ||
| mcr-3 (1) | F3 | CATTACCAATATTGCTTGTTGC | This study |
| B3 | TTGGCTGGAACAATCTCAC | ||
| FIP | GCTAACGCCTCATTTTGATTGGTTGCACTTCTTATCGCACTTA | ||
| BIP | TCAAAGGGATTCTAACTCGTGCGCAATAACCGCAATCACTAT | ||
| LF | TCATTGTGTAACTAACGATTGC | ||
| LB | CCTATCGATGTTTGCATCACTT | ||
| mcr-3 (2) | F3 | CATTACCAATATTGCTTGTTGC | This study |
| B3 | TTGGCTGGAACAATCTCAC | ||
| FIP | GCTAACGCCTCATTTTGATTGGTGCACTTCTTATCGCACTTAG | ||
| BIP | TCAAAGGGATTCTAACTCGTGCGCAATAACCGCAATCACTAT | ||
| LF | TCATTGTGTAACTAACGATTGC | ||
| LB | CCTATCGATGTTTGCATCACTT | ||
| mcr-3 (3) | F3 | CATTACCAATATTGCTTGTTGC | This study |
| B3 | TTGGCTGGAACAATCTCAC | ||
| FIP | GCTAACGCCTCATTTTGATTGGGCACTTCTTATCGCACTTAGT | ||
| BIP | TCAAAGGGATTCTAACTCGTGCGCAATAACCGCAATCACTAT | ||
| LF | TCATTGTGTAACTAACGATTGC | ||
| LB | CCTATCGATGTTTGCATCACTT | ||
| mcr-4 (1) | F3 | TGAGTTAAGGCGTTACATTGT | This study |
| B3 | CGCATGAGCTAGTATCGTTAA | ||
| FIP | TTACGACTGGCATTCTTCGCACTATTTGCAGACGCCCAT | ||
| BIP | AGTGGTTGTTGTGGGTGAAACTAGCATTGGTTGGCTTGTTA | ||
| LF | TCTAGGCCAAGTTGTTGGTATT | ||
| LB | CGCGCTCAATGAGCTATCA | ||
| mcr-4 (2) | F3 | TGTTCGAAACAACAGTGAGT | This study |
| B3 | CGCATGAGCTAGTATCGTTAA | ||
| FIP | TTACGACTGGCATTCTTCGCATTGAGCACTATTTGCAGACG | ||
| BIP | AGTGGTTGTTGTGGGTGAAACTAGCATTGGTTGGCTTGTTA | ||
| LF | GCCAAGTTGTTGGTATTCCATG | ||
| LB | CGCGCTCAATGAGCTATCA | ||
| mcr-4 (3) | F3 | CATGGAATACCAACAACTTGG | This study |
| B3 | CGGCGAGGATCATAGTCT | ||
| FIP | AGTTTCACCCACAACAACCACTAGATGCGAAGAATGCCAG | ||
| BIP | CAAGCCAACCAATGCTCATACCTGCCGCATGAGCTAGTAT | ||
| LF | TAGGTTTAGTGTTCGGGTTACG | ||
| LB | GGGCTGATTGCGTTTAACG | ||
| mcr-5 (1) | F3 | CAATGGAGAATGCTGCCCTA | This study |
| B3 | GCGTGGGTATCAGCACATC | ||
| FIP | AGCCCGTTCGTAAAACCCTGACCTTGTTGGTTGCAGCCGT | ||
| BIP | AGCGGTAATGATGCGCAGCGCATGACTGGCCACAGACC | ||
| LF | CTCGCAATCCACCACACGGAT | ||
| LB | TGGCGCTCTCGCCATGA | ||
| mcr-5 (2) | F3 | ATGGATGTGCTGATACCCAC | This study |
| B3 | AGTACGAGAGCACGAGGAC | ||
| FIP | CGAATGCCCGAGATGACGTAGTCTTCGTGAAAACAAGCCGC | ||
| BIP | GACTGAACAGGCGTCATCGTCACCTTGTTCTTGAGGCCCTC | ||
| LF | GCAGGAGTGATCAAATAGCGAA | ||
| LB | GCAGACGAAGCAAGGGAAG | ||
| mcr-5 (3) | F3 | GCTGCCCTACTTGTTGGTTG | This study |
| B3 | CGAAGCGGCTTGTTTTCAC | ||
| FIP | TACCGCTTGTTTCCAGCCCGCAGCCGTATCCGTGTGGT | ||
| BIP | CTGGCGCTCTCGCCATGATTGAAGCGTGGGTATCAGCAC | ||
| LF | ACCCTGACTCTCGCAATCC | ||
| LB | TCCATGGGTCTGTGGCC |