Figure 1.

Growth inhibition of Candida albicans and Saccharomyces cerevisiae after treatment with 5 nm AgNPs. (A) Growth kinetics and (C) spot assay of C. albicans in either the absence or presence of 5 nm or 100 nm AgNPs (2, 20, and 50 μg/mL). The C. albicans culture was diluted with fresh Sabouraud dextrose broth and cultured for 12 hours at 37°C. Spot assay was performed by inoculation of 10-fold serial dilutions of 12-hour culture on the Sabouraud dextrose agar plates. (B) Growth kinetics and (D) spot assay of S. cerevisiae in either the absence or presence of 5 nm or 100 nm AgNPs. The S. cerevisiae culture was diluted with fresh YPD broth and cultured for 20 hours at 30°C. Spot assay was performed by inoculation of 10-fold serial dilutions of 20-hour culture on the YPD agar plates. Cell growth was measured at 600 nm. Control consists of yeast cells not treated with AgNPs. Data are presented as mean ± SD from two independent experiments performed in triplicate. **P<0.005, ***P<0.001 vs control and vs cells treated with 100 nm AgNPs.

Abbreviations: AgNPs, silver nanoparticles; BrPA, 3-bromopyruvate; YPD, 1% yeast extract, 2% peptone, and 2% dextrose.