Figure 3.

G1 phase delay in Candida albicans and Saccharomyces cerevisiae in the presence of 5 nm AgNPs. (A) Representative cell cycle analysis histogram of C. albicans either treated (N +) or not treated (N –) with 15 μg/mL nocodazole at the indicated time-points. Representative histograms of (B) C. albicans and (C) S. cerevisiae treated with 0, 2, 20, and 50 μg/mL 5 nm AgNPs for 2 hours and then analyzed by propidium iodide labeling. The left peak represents G1 phase and a single copy of DNA per cell, and the right peak represents G2 phase and two copies of DNA. G1 phase cell proportion of (D) C. albicans and (E) S. cerevisiae in the presence of 5 nm or 100 nm AgNPs at the indicated time-points. Data are presented as mean ± SD from two independent experiments performed in triplicate. *P<0.05, **P<0.005.

Abbreviations: AgNPs, silver nanoparticles; ns, not significant.