3.

Knockdown of cancer-derived IgG (CIgG) inhibited SW480 cell proliferation, invasion and migration. (A) Western blot showed that CIgG was down-regulated in colorectal cancer cells after transfection with siRNAs; (B) Knockdown of non-B-IgG inhibited proliferation of SW480 cells. The relative growth (OD values) was assessed with cell counting Kit-8 assays. *, P<0.05; **, P<0.01. siNC, normal control siRNA; siRNA, small interfering RNA; OD, optical density; (C) Wound healing assay was performed on SW480 cells treated with siRNAs or siNC. The wound breadth was measured at the indicated time points; (D) SW480 cells were treated with siRNAs or siNC for 48 h. The plates were stained and photographed 14 d later. Colony percentages were calculated; (E) CIgG inhibition suppresses SW480 cell migration and invasion; (F) Suppression of tumor growth by inhibition of CIgG in SW480 cell subcutaneous xenografts. Scale bar, 5 mm.