4.

Hypoxia promotes translocation of hexokinase (HK) II into mitochondria and increases phosphorylation of the alpha subunit of pyruvate dehydrogenase (PDHA1). (A) HeLa cells were incubated under normoxia or hypoxia conditions. Endogenous proteins of cytoplasm and mitochondria were isolated, respectively and endogenous HK I, HKII, PDHA1 and PDHK1 were determined and quantified; (B) P-S293 levels of endogenous PDHA1 of each cell cultured under normoxia or hypoxia were determined and quantified; (C) HA-tagged HKII was overexpressed in HeLa cells under normoxia or hypoxia. P-S293 levels of endogenous PDHA1 of cultures with or without 3-bromopyruvate (3BP; 5 mmol/L) were determined.