Table 1.

General Principles for activity screening strategies

Technology	Mode	Principle	Application examples
Cell based assays	In vitro	Metabolite-induced cellular phenotype (e.g. cell migration) is quantified	140
Expression screening	In vitro	Metabolite-induced protein or transcript expression is quantified	14
Metabolite binding screening on protein arrays	In vitro	Metabolite-protein interactions are profiled	83,84
Chemical proteomics	In vitro	Metabolite-protein interactions are profiled	83–86
In vivo administration and phenotype screening	In vivo	Metabolite-induced molecular and patho-physiological phenotypes of model organisms are quantified	141
Mathematical modeling	In silico	Mass balance or neuronal networks are used to predict most active metabolites	91
Cognitive computing	In silico	Active metabolites are predicted based on previous studies	142