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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Dalton Trans. 2019 Jul 2;48(26):9654–9662. doi: 10.1039/c9dt00642g

Figure 4 |. Fluconazole does not compete with chelators BCA or Fz for Cu(I) as determined by UV-Vis spectroscopy.

Figure 4 |

Fluconazole does not pull Cu(I) away from the pre-formed [CuI(BCA)2]3– complex (a), or from the weaker pre-formed [CuI(Fz)2]3- complex (b). Presence of fluconazole in solution does not prevent formation of the [CuI(Fz)2]3- complex (c). CuCl2 was used as the source of Cu(II), and ascorbate was added to generate Cu(I) in situ. Conditions: [BCA, Fz] = 100 µM, [CuCl2] = 40 µM, [Ascorbate] = 1 mM, [FluC] = 1 mM in 50 mM HEPES, pH 7.4.