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(A) SDS–PAGE showing purified Pol δ in the presence (left) or absence (right) of PCNA. Asterisk denotes baculovirus PCNA that copurifies with Pol δ. (B) Scheme of primer extension assay. Grey circle indicates 5′-fluorescein amidite label. (C, D) Time-course analysis of primer extension with 2 nM of the indicated enzymes in the absence (C) or presence (D) of PCNA. Products were resolved on a denaturing polyacrylamide gel. MW, molecular weight.
