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. Author manuscript; available in PMC: 2019 Jul 8.
Published in final edited form as: Cell Rep. 2018 Oct 23;25(4):1018–1026.e4. doi: 10.1016/j.celrep.2018.09.074

Figure 3. D2HG Production and Secretion Increase NADPH Demands in IDH1+/R132C Cells.

Figure 3.

(A) NADPH consumption fluxes by lipid synthesis and 2HG production in fibrosarcoma panel.

(B) Atom transition map of [3-2H]glucose.

(C) Contribution of oxPPP to cytosolic NADPH in fibrosarcoma panel.

(D) 2HG abundance in parental HT1080 cells upon treatment with 10 mM AGI-5198.

(E) Contribution of oxPPP to cytosolic NADPH in parental HT1080 cells with 10 mM AGI-5198.

(F) Contribution of oxPPP to cytosolic NADPH in non-native IDH1-R132H engineered HCT116 cells.

In (A), (C), (E), and (F), data are plotted as mean ± 95% confidence interval (CI). *Statistical significance by non-overlapping confidence intervals. In (D), data are plotted as mean ± SEM. Unless indicated, all data represent biological triplicates. See also Figure S2.