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. Author manuscript; available in PMC: 2019 Jul 8.
Published in final edited form as: Oral Dis. 2018 Apr 24;25(2):403–415. doi: 10.1111/odi.12840

Figure 3: Culturing human and mouse submandibular gland cells.

Figure 3:

Human (A-D) and mouse (E-H) SMG tissue was obtained and dissociated (as detailed in Fig. 2). Suspended cells and cell clusters were then plated on Laminin-111 (L1) gel in 8-well chambered coverglass slides and grown for 6 (A, E), 14 (B, F), and 22 (C, G) days. TO-PRO™−3 iodide and phalloidin were used to stain nuclei blue and F-actin red, respectively (A, B, C, E, F, G). Human (D) and mouse (H) SMG cells were then cultured on Laminin-111 rich gel for six days and captured in histogel for fixing, sectioning, and staining with H&E. Yellow dotted lines are indicative of lumen formation (as evidenced by a lack of nuclei) and white arrowheads point to apically localized F-actin (A, B, C, F).