Fig 3. EF-P is required for maximal Phag expression.

β-galactosidase activity reported in Miller Units (MU) of a transcriptional fusion of lacZ to the σD-dependent flagellin promoter Phag. The levels of expression in WT and the efp mutant are indicated by dashed lines for comparison. Data represent the average of three biological replicates, error bars indicate the standard deviation, and the raw values can be found in S2 Table. The following strains were used to generate this panel: WT (DK5457), efp (DK5458), swrA (DK7032), efp swrA (DK7033), flgM (DK7074), efp flgM (DK7078), swrA flgM (DK7076), and efp swrA flgM (DK7077), efp fliYsoe (DK7049), and efp nusGsoe (DK7050).