Figure 7.

Suspended fiber patterns guide (U87) cell aggregate morphology. (a–d) Suspended fiber devices with different in-plane patterns provide topographic guidance for fusion and expansion of suspended cell aggregates over 10 days. A viability assay highlights the survival of cell aggregates cultured on suspended fiber devices at (e, f) day 1 and (g–i) day 10. (g1, g2, h1, h2) Separate live and dead intensities recorded from an aggregate-on-string and suspended cell sheet cultured over 10 days. (i1) 3D reconstruction of a z-stack acquired for a suspended aggregate grown on a suspended fiber device with fibers offset by 30° (i).