FIG 2.

Top escapees identified by RNA-seq behave like SRE-containing transcripts. (A) Total RNA was extracted from unreactivated or reactivated KSHV-positive iSLK.219 cells and subjected to RT-qPCR to measure endogenous levels of the top candidates identified by RNA-seq. (B) 293T cells were transfected with an empty vector (mock) or a plasmid expressing each of the viral endonucleases color coded on the right. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure endogenous RNA levels. (C) 293T cells were transfected with an empty vector (mock) or a plasmid expressing nsp1. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure endogenous RNA levels. ***, P < 0.001.