FIG 4.

C19ORF66 restricts KSHV reactivation. (A) Diagram outlining the fluorescence pattern of iSLK.219 cells. iSLK.219 cells were either treated with siRNAs targeting C19ORF66 (or control nontarget siRNAs) for 48 h (B) or transfected with a Flag-tagged C19ORF66 (C). Cells were then reactivated with doxycycline and sodium butyrate and lysed, and lysates were resolved on SDS-PAGE and Western blotted with the indicated antibodies. (D) Cells were treated with the indicated siRNA or transfected with C19ORF66 (overexpression [OE]) and were checked for reactivation efficiency by monitoring the expression of GFP and RFP. Quantification of RFP-positive cells is to the right. Values represent at least four independent views of the infected cells. **, P < 0.01.